AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE Manual do Utilizador descarregar pdf (Página 15)

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Chapter 2 Designing Primers and Probes for Quantification Assays

Overview

8 Primer Express Software 3.0 Getting Started Guide

Notes

Overview

About This

Chapter

This chapter provides information on using Primer Express Software Version 3.0 to

automatically design primers and probes for quantification assays (including SYBR

Green Dye Assays) using default parameters. It also includes information on how to

manually design primers and probes to obtain customized results.

Workflow

Figure 1. Quantification workflow

YesNo

Open or Create a

Quantification Document

Load a DNA Sequence File

Find Primers and Probes

View Results

Primers and Probes

Found?

Order Primers

and Probes

Manually Design

the Probe

Manually Design

the Primers

Desired

Primers and Probes

Found?

Edit Default

Parameters

Order Primers

and Probes

YesNo

Quantification Workflow

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